7XZ9
Mutant (E236A) of the N-terminal domain of fucoidan lyase FdlA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 193.15 |
Detector technology | PIXEL |
Collection date | 2021-12-30 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 |
Unit cell lengths | 53.844, 58.005, 87.940 |
Unit cell angles | 90.42, 101.60, 91.98 |
Refinement procedure
Resolution | 34.810 - 1.540 |
R-factor | 0.1648 |
Rwork | 0.164 |
R-free | 0.18790 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7xzf |
RMSD bond length | 0.006 |
RMSD bond angle | 0.887 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 86.130 | 1.560 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmerge | 0.164 | |
Rmeas | 0.204 | |
Number of reflections | 148952 | 4591 |
<I/σ(I)> | 5.3 | |
Completeness [%] | 96.6 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291.15 | 0.1 M Tris pH8.5, 0.2 M lithium sulfate, 30% (w/v) polyethylene glycol (PEG) 4000 |