7XZ7

Mutant (D137A) of the N-terminal domain of fucoidan lyase FdlA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	193.15
Detector technology	PIXEL
Collection date	2021-11-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	54.551, 58.386, 88.287
Unit cell angles	90.17, 101.92, 92.25

Refinement procedure

Resolution	58.330 - 1.980
R-factor	0.1582
Rwork	0.156
R-free	0.19750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xzf
RMSD bond length	0.018
RMSD bond angle	1.448
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.330	2.000
High resolution limit [Å]	1.980	1.980
Rmerge	0.069	0.889
Rmeas	0.092
Rpim	0.047
Number of reflections	67747	2754
<I/σ(I)>	6.7
Completeness [%]	90.9	92
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291.15	0.1M Tris, pH8.5, 0.2M Li2SO4, 30% (w/v) polyethylene glycol (PEG) 4000