7XZ3
Crystal structure of the Type I-B CRISPR-associated protein, Csh2 from Thermobaculum terrenum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 11C |
Synchrotron site | PAL/PLS |
Beamline | 11C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 1 |
Unit cell lengths | 30.946, 54.432, 96.364 |
Unit cell angles | 106.50, 91.49, 90.09 |
Refinement procedure
Resolution | 19.895 - 1.889 |
R-factor | 0.1788 |
Rwork | 0.177 |
R-free | 0.22080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7kha |
RMSD bond length | 0.003 |
RMSD bond angle | 0.688 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 1.930 |
High resolution limit [Å] | 1.889 | 5.130 | 1.900 |
Rmerge | 0.067 | 0.039 | 0.557 |
Rmeas | 0.079 | 0.045 | 0.667 |
Rpim | 0.042 | 0.024 | 0.362 |
Number of reflections | 46406 | 2327 | 2320 |
<I/σ(I)> | 5.8 | ||
Completeness [%] | 96.7 | 98.2 | 95.4 |
Redundancy | 3.4 | 3.6 | 3.2 |
CC(1/2) | 0.998 | 0.782 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 7 % (w/v) Polyethylene glycol 3350, 2 % (v/v) Tacsimate pH 5.0, 0.1 M Sodium citrate pH 5.6 |