7XZ1
TRIM E3 ubiquitin ligase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00933 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 235.921, 235.921, 158.814 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.860 - 5.200 |
R-factor | 0.2709 |
Rwork | 0.266 |
R-free | 0.31520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7xv2 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.691 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 5.290 |
High resolution limit [Å] | 5.200 | 5.200 |
Rmerge | 0.135 | |
Number of reflections | 9007 | 452 |
<I/σ(I)> | 49.84 | |
Completeness [%] | 99.9 | |
Redundancy | 28.8 | |
CC(1/2) | 0.992 | 0.523 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100 mM Hepes-NaOH pH 7.5, 250 mM MgCl2, 37% (w/v) 5/4 PO/OH |