7XZ0

TRIM E3 ubiquitin ligase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	239.728, 72.967, 83.764
Unit cell angles	90.00, 97.60, 90.00

Refinement procedure

Resolution	37.740 - 3.280
R-factor	0.2588
Rwork	0.254
R-free	0.30780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xv2
RMSD bond length	0.003
RMSD bond angle	0.650
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.340
High resolution limit [Å]	3.280	3.280
Number of reflections	22376	1110
<I/σ(I)>	21.5
Completeness [%]	98.8
Redundancy	3.9
CC(1/2)	0.995	0.558

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	100 mM TRIS-HCl pH 8.5, 8% (v/v) PEG 8000