7XYZ
TRIM E3 ubiquitin ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 |
| Unit cell lengths | 174.375, 174.375, 224.941 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.790 - 4.620 |
| R-factor | 0.314 |
| Rwork | 0.312 |
| R-free | 0.34840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7DEW |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.028 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.680 |
| High resolution limit [Å] | 4.600 | 4.600 |
| Number of reflections | 40138 | 1552 |
| <I/σ(I)> | 7.53 | |
| Completeness [%] | 94.9 | |
| Redundancy | 4.4 | |
| CC(1/2) | 0.992 | 0.985 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100 mM MES-NaOH pH 6.5, 200 mM MgCl2, 0.5% (w/v) polyvinylpyrrolidone K15, 30% (w/v) 5/4 PO/OH |
