7XYX
Crystal structure of ZYG11B bound to CFLH degron
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-03 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.979183 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.777, 97.369, 122.888 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.400 - 2.870 |
R-factor | 0.2049 |
Rwork | 0.202 |
R-free | 0.26380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ep1 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.480 | 2.970 |
High resolution limit [Å] | 2.870 | 2.870 |
Rmerge | 0.259 | 1.507 |
Number of reflections | 15037 | 1466 |
<I/σ(I)> | 13.3 | |
Completeness [%] | 98.5 | |
Redundancy | 13.3 | |
CC(1/2) | 0.046 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M MES (pH 6.5), 1.6 M MgSO4 |