7XYW
Crystal structure of ZYG11B bound to AFLH degron
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-03 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.979183 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 52.880, 97.266, 122.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.490 - 2.500 |
R-factor | 0.2285 |
Rwork | 0.226 |
R-free | 0.27520 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ep1 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.450 | 2.630 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.109 | 1.413 |
Number of reflections | 21062 | 3269 |
<I/σ(I)> | 15.6 | |
Completeness [%] | 92.2 | |
Redundancy | 12.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M MES (pH 6.5), 1.6 M MgSO4 |