7XYT

Crystal structure of ZER1 bound to AFLH degron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-03
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	63.979, 138.273, 69.510
Unit cell angles	90.00, 117.38, 90.00

Refinement procedure

Resolution	34.610 - 1.500
R-factor	0.1972
Rwork	0.196
R-free	0.22550
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ep3
RMSD bond length	0.006
RMSD bond angle	0.816
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	138.270	1.540
High resolution limit [Å]	1.500	1.500
Rmerge	0.118	1.568
Number of reflections	170861	12605
<I/σ(I)>	8.97
Completeness [%]	99.9
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M NH4F, 20% PEG3350