7XXZ
Macaca mulatta galectin-10/Charcot-Leyden crystal protein with glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 48.720, 48.720, 259.204 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.540 - 1.830 |
| R-factor | 0.1801 |
| Rwork | 0.177 |
| R-free | 0.20770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xrg |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.540 | 1.870 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.100 | 0.650 |
| Number of reflections | 17232 | 1034 |
| <I/σ(I)> | 14 | |
| Completeness [%] | 99.6 | |
| Redundancy | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG |
