7XXZ
Macaca mulatta galectin-10/Charcot-Leyden crystal protein with glycerol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-01-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97930 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 48.720, 48.720, 259.204 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.540 - 1.830 |
R-factor | 0.1801 |
Rwork | 0.177 |
R-free | 0.20770 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xrg |
Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.540 | 1.870 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.100 | 0.650 |
Number of reflections | 17232 | 1034 |
<I/σ(I)> | 14 | |
Completeness [%] | 99.6 | |
Redundancy | 9.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG |