7XXW
Macaca fascicularis galectin-10/Charcot-Leyden crystal protein with glycerol
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-31 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97930 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 48.862, 48.862, 259.472 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.590 - 1.630 |
R-factor | 0.1794 |
Rwork | 0.177 |
R-free | 0.20540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xrg |
Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.960 | 1.660 |
High resolution limit [Å] | 1.630 | 1.630 |
Rmerge | 0.065 | 0.469 |
Number of reflections | 24277 | 1145 |
<I/σ(I)> | 26.5 | |
Completeness [%] | 99.9 | |
Redundancy | 17.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG |