7XXE

Crystal structure of spFft3 C-terminal truncation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-12-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.978
Spacegroup name	P 63
Unit cell lengths	99.649, 99.649, 130.550
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.550 - 4.202
R-factor	0.3108
Rwork	0.308
R-free	0.33690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gn1
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.550	4.270
High resolution limit [Å]	4.202	4.202
Number of reflections	5377	250
<I/σ(I)>	34.1
Completeness [%]	95.5
Redundancy	20.3
CC(1/2)	0.976	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M HEPES pH 7.0, 30% v/v Jeffamine ED-2001 pH 7.0