7XX5
Crystal Structure of Nucleosome-H1.3 Linker Histone Assembly (sticky-169a DNA fragment)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 98.15 |
Detector technology | PIXEL |
Collection date | 2015-09-22 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 105.071, 101.736, 218.399 |
Unit cell angles | 90.00, 103.52, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.210 | 3.370 |
High resolution limit [Å] | 3.190 | 3.190 |
Number of reflections | 73544 | 10394 |
<I/σ(I)> | 10.2 | 1.4 |
Completeness [%] | 98.5 | 96.1 |
Redundancy | 3.3 | 3.2 |
CC(1/2) | 0.998 | 0.532 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 40-45mM CaCl2, 25mM KCl, 10mM Na-acetate (pH 4.5) |