7XX1
Crystal structure of SARS-CoV-2 N-NTD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-04-09 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97936 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 33.466, 76.783, 109.702 |
Unit cell angles | 90.00, 91.79, 90.00 |
Refinement procedure
Resolution | 44.660 - 1.900 |
R-factor | 0.2119 |
Rwork | 0.210 |
R-free | 0.25840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2ofz |
RMSD bond length | 0.008 |
RMSD bond angle | 1.431 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.660 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.064 | 0.590 |
Number of reflections | 43619 | 2784 |
<I/σ(I)> | 10.6 | |
Completeness [%] | 99.6 | |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | Zinc chloride, MES, PEG 6000 |