7XX1
Crystal structure of SARS-CoV-2 N-NTD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-2 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-09 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97936 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.466, 76.783, 109.702 |
| Unit cell angles | 90.00, 91.79, 90.00 |
Refinement procedure
| Resolution | 44.660 - 1.900 |
| R-factor | 0.2119 |
| Rwork | 0.210 |
| R-free | 0.25840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ofz |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.431 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.660 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.064 | 0.590 |
| Number of reflections | 43619 | 2784 |
| <I/σ(I)> | 10.6 | |
| Completeness [%] | 99.6 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | Zinc chloride, MES, PEG 6000 |
