7XWX
Crystal structure of SARS-CoV-2 N-CTD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-04-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 41.456, 286.665, 164.342 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.650 - 3.000 |
| R-factor | 0.2217 |
| Rwork | 0.219 |
| R-free | 0.26810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cjr |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.398 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.650 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.224 | 0.856 |
| Number of reflections | 20290 | 3247 |
| <I/σ(I)> | 12 | |
| Completeness [%] | 99.6 | |
| Redundancy | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | NaNO3, Na2HPO4, (NH4)2SO4, Tris(base), Bicine, PEG MME500, PEG 20000, 1,8-ANS |
