7XWS
Crystal structure of Wild Type Cypovirus Polyhedra produced by cell-free protein synthesis with small volume
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-18 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | I 2 3 |
Unit cell lengths | 103.600, 103.600, 103.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.295 - 1.950 |
Rwork | 0.166 |
R-free | 0.21570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gqm |
RMSD bond length | 0.007 |
RMSD bond angle | 1.442 |
Data reduction software | XDS |
Data scaling software | CrystFEL |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.960 |
High resolution limit [Å] | 1.950 | 1.950 |
Number of reflections | 13641 | 309 |
<I/σ(I)> | 7.3 | 1.23 |
Completeness [%] | 100.0 | 100 |
Redundancy | 447.7 | |
CC(1/2) | 0.991 | 0.671 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | SMALL TUBES | 293 | cell-free crystallization |