7XWS
Crystal structure of Wild Type Cypovirus Polyhedra produced by cell-free protein synthesis with small volume
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 103.600, 103.600, 103.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.295 - 1.950 |
| Rwork | 0.166 |
| R-free | 0.21570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gqm |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.442 |
| Data reduction software | XDS |
| Data scaling software | CrystFEL |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.960 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 13641 | 309 |
| <I/σ(I)> | 7.3 | 1.23 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 447.7 | |
| CC(1/2) | 0.991 | 0.671 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | SMALL TUBES | 293 | cell-free crystallization |
