7XWD
Apo-AtPRT6 UBR box
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2020-02-08 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 87.165, 87.165, 74.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.583 - 2.396 |
| R-factor | 0.2236 |
| Rwork | 0.219 |
| R-free | 0.26850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6lhn |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.160 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.583 | 2.482 |
| High resolution limit [Å] | 2.396 | 2.396 |
| Number of reflections | 5882 | 573 |
| <I/σ(I)> | 14.06 | |
| Completeness [%] | 99.9 | |
| Redundancy | 2 | |
| CC(1/2) | 0.997 | 0.815 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 1.6M ammonium sulfate, 0.1 M sodium HEPES pH 7.5, 0.1 M NaCl |
