7XVW
Structure of neuraminidase from influenza B-like viruses derived from spiny eel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-07 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.97918 |
Spacegroup name | I 4 |
Unit cell lengths | 126.085, 126.085, 110.432 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.870 - 1.940 |
R-factor | 0.1583 |
Rwork | 0.157 |
R-free | 0.19030 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7xvr |
RMSD bond length | 0.007 |
RMSD bond angle | 0.924 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.040 |
High resolution limit [Å] | 1.940 | 1.940 |
Rpim | 0.102 | 0.446 |
Number of reflections | 60070 | |
<I/σ(I)> | 8.3 | |
Completeness [%] | 93.7 | |
Redundancy | 13.9 | |
CC(1/2) | 0.993 | 0.815 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 291 | 4.0 M Potassium formate, 0.1 M BIS-TRIS propane pH 9.0, 2% w/v Polyethylene glycol monomethyl ether 2,000. NA crystals were soaked in 10 mM zanamivir, oseltamivir and peramivir for 30 min. |