7XVR

Structure of neuraminidase from influenza B-like viruses derived from spiny eel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-30
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	I 4
Unit cell lengths	126.513, 126.513, 111.173
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.560 - 2.400
R-factor	0.1569
Rwork	0.155
R-free	0.19730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3k36
RMSD bond length	0.008
RMSD bond angle	0.994
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rpim	0.081	0.384
Number of reflections	35110
<I/σ(I)>	10.9	4
Completeness [%]	100.0
Redundancy	6.1
CC(1/2)	0.979	0.811

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	291	4.0 M Potassium formate, 0.1 M BIS-TRIS propane pH 9.0, 2% w/v Polyethylene glycol monomethyl ether 2,000.