7XV2

TRIM E3 ubiquitin ligase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-11
Detector	RAYONIX MX-225
Wavelength(s)	0.9796
Spacegroup name	C 1 2 1
Unit cell lengths	73.295, 110.171, 95.936
Unit cell angles	90.00, 100.80, 90.00

Refinement procedure

Resolution	27.410 - 2.750
R-factor	0.2101
Rwork	0.205
R-free	0.25840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3kb5
RMSD bond length	0.009
RMSD bond angle	1.113
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.800
High resolution limit [Å]	2.750	2.750
Rmerge	0.087
Number of reflections	19188	948
<I/σ(I)>	27.3	1.3
Completeness [%]	97.6	98.9
Redundancy	4.1
CC(1/2)	0.990	0.806

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	100 mM Tris-HCl pH 8.5, 30% (v/v) PEG 400