7XV1

Crystal structure of RPA70N-HelB fusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 41 21 2
Unit cell lengths	50.333, 50.333, 126.481
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.680 - 1.800
R-factor	0.1977
Rwork	0.197
R-free	0.21580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eay
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20rc1_4392)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.680	1.864
High resolution limit [Å]	1.800	1.800
Rmerge	0.062	0.483
Rpim	0.019	0.140
Number of reflections	28713	2524
<I/σ(I)>	26.49
Completeness [%]	99.8	100
Redundancy	12.3
CC(1/2)	0.998	0.982

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	20% PEG3350 200 mM Ammonium Acetate