7XUX
Crystal structure of a FIC domain containnig protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-06-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979813 |
Spacegroup name | C 2 2 2 |
Unit cell lengths | 115.464, 282.767, 73.571 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.860 - 2.780 |
R-factor | 0.231 |
Rwork | 0.229 |
R-free | 0.25740 |
Structure solution method | MAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.506 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 141.380 | 2.850 |
High resolution limit [Å] | 2.780 | 2.780 |
Rmerge | 0.206 | 4.060 |
Number of reflections | 406690 | 30176 |
<I/σ(I)> | 0.8 | |
Completeness [%] | 100.0 | |
Redundancy | 13.2 | |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 289 | 0.1 M MES pH 6.5, 25% Glycerol |