7XUW

Crystal structure of RPA70N-BLMp2 fusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 41 21 2
Unit cell lengths	62.476, 62.476, 69.497
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.940 - 1.800
R-factor	0.1941
Rwork	0.193
R-free	0.21740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5eay
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20rc1_4392)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.940	1.880
High resolution limit [Å]	1.800	1.800
Rmerge	0.131	0.767
Rpim	0.039	0.222
Number of reflections	24353	2918
<I/σ(I)>	13.6
Completeness [%]	99.9	100
Redundancy	12.3
CC(1/2)	0.996	0.954

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	278	8% PEG 4000