7XUV
Crystal structure of RPA70N-RMI1 fusion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97852 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.930, 50.263, 52.308 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.240 - 1.600 |
R-factor | 0.1884 |
Rwork | 0.187 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5eay |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20rc1_4392) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.240 | 1.657 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.069 | 0.342 |
Rpim | 0.032 | 0.158 |
Number of reflections | 22637 | 2779 |
<I/σ(I)> | 13.8 | |
Completeness [%] | 89.4 | |
Redundancy | 4.6 | |
CC(1/2) | 0.997 | 0.953 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 278 | 30% PEG 2000 MME |