7XUT
Crystal structure of RPA70N-WRN fusion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97915 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 32.936, 58.609, 111.786 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.590 - 1.600 |
R-factor | 0.181 |
Rwork | 0.179 |
R-free | 0.21410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5eay |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20rc1_4392) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.590 | 1.657 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.097 | 0.152 |
Rpim | 0.042 | 0.064 |
Number of reflections | 54591 | 2870 |
<I/σ(I)> | 13.82 | |
Completeness [%] | 99.8 | 100 |
Redundancy | 6.2 | |
CC(1/2) | 0.992 | 0.980 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 278 | 20% PEG3350, 200 mM ammonium sulfate |