7XTF
Crystal Structure of PL-5 family polysaccharide lyase PanPL-Y226F mutant from Pandoraea apista in apo form at pH5.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-09-29 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.96770 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.073, 85.682, 91.565 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.840 - 1.730 |
R-factor | 0.1818 |
Rwork | 0.180 |
R-free | 0.22210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7wxj |
RMSD bond length | 0.009 |
RMSD bond angle | 0.892 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.783 | 1.755 |
High resolution limit [Å] | 1.726 | 1.726 |
Rmerge | 0.066 | 0.681 |
Rpim | 0.036 | 0.352 |
Number of reflections | 30829 | 1551 |
<I/σ(I)> | 14 | 2.2 |
Completeness [%] | 97.8 | 99.8 |
Redundancy | 4.3 | 4.6 |
CC(1/2) | 0.997 | 0.793 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295.15 | 0.1M Bis-Tris pH5.5, 25% w/v PEG 3350 |