7XT3

Crystal Structure of Hepatitis virus A 2C protein 128-335 aa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-26
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9790
Spacegroup name	P 21 21 21
Unit cell lengths	50.165, 54.687, 85.666
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.290 - 2.150
R-factor	0.2245
Rwork	0.223
R-free	0.25760
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.290	43.290	2.280
High resolution limit [Å]	2.150	6.400	2.150
Rmerge	0.150	0.035	1.789
Rmeas	0.172	0.040	2.037
Total number of observations	106315
Number of reflections	24694	948	4010
<I/σ(I)>	7.67	33.35	0.81
Completeness [%]	99.9	99.3	99.6
Redundancy	4.305	4.504	4.398
CC(1/2)	0.995	0.999	0.321

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.45 mM Ammonium phosphate monobasic