7XSW
Structure of SARS-CoV-2 antibody S309 with GX/P2V/2017 RBD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-18 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.978530 |
Spacegroup name | P 1 |
Unit cell lengths | 54.639, 73.599, 105.615 |
Unit cell angles | 110.53, 92.08, 97.05 |
Refinement procedure
Resolution | 48.540 - 3.300 |
R-factor | 0.2808 |
Rwork | 0.277 |
R-free | 0.32160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7r6x |
RMSD bond length | 0.002 |
RMSD bond angle | 0.543 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.500 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmeas | 3.421 | 3.303 |
Number of reflections | 40975 | 6611 |
<I/σ(I)> | 1.9 | |
Completeness [%] | 89.0 | |
Redundancy | 4.94 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2M magnesium chloride hexahydrate, 0.1M MES (pH 6.0), 20% w/v PEG 6000 |