7XSJ

The structure of the Mint1/Munc18-1/syntaxin-1 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-22
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 4 21 2
Unit cell lengths	156.238, 156.238, 78.857
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.870 - 3.200
R-factor	0.2367
Rwork	0.235
R-free	0.26970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4jeh
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.310
High resolution limit [Å]	3.200	6.890	3.200
Rmerge	0.109	0.043	0.837
Rmeas	0.111	0.043	0.853
Rpim	0.022		0.167
Total number of observations	422820
Number of reflections	16652	1812	1630
<I/σ(I)>	9.2
Completeness [%]	100.0	99.7	100
Redundancy	25.4
CC(1/2)		1.000	0.968

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		297	0.2M Sodium Malonate, pH 7.0, 20% (v/v) PEG 3350