7XS2

Monomer structure of HtrA from Helicobacter pylori

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 64 2 2
Unit cell lengths	146.199, 146.199, 94.231
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	63.306 - 2.150
R-factor	0.2443
Rwork	0.243
R-free	0.26970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pv2
RMSD bond length	0.008
RMSD bond angle	1.129
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.590	2.270
High resolution limit [Å]	2.150	2.150
Rpim		0.421
Number of reflections	32753	4671
<I/σ(I)>	23.8
Completeness [%]	100.0
Redundancy	35.7
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.4	293	0.1 M sodium citrate, pH 3.4, 2.7 M ammonium sulphate