7XRZ

Crystal structure of BRIL and SRP2070_Fab complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00004
Spacegroup name	C 1 2 1
Unit cell lengths	93.828, 75.239, 152.231
Unit cell angles	90.00, 99.67, 90.00

Refinement procedure

Resolution	42.740 - 2.100
R-factor	0.2164
Rwork	0.214
R-free	0.26840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m6t 1f58
RMSD bond length	0.004
RMSD bond angle	1.319
Data reduction software	XDS
Data scaling software	Aimless (0.5.21)
Phasing software	PHASER (2.6.1)
Refinement software	REFMAC (5.8.0232)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.640	48.640	2.160
High resolution limit [Å]	2.100	9.150	2.100
Rmerge	0.063	0.019	0.645
Rmeas	0.080	0.024	0.815
Rpim	0.049	0.015	0.490
Total number of observations	134803	1787	10143
Number of reflections	57858	705	4489
<I/σ(I)>	10.8	44.2	1.5
Completeness [%]	94.9	90	94.7
Redundancy	2.3	2.5	2.3
CC(1/2)	0.996	0.997	0.568

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277.15	PEG 6000, Tris-HCl