7XRX

insulin-cleaving membrane protease-ICMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	80
Detector technology	CCD
Collection date	2017-12-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	87.927, 78.141, 99.916
Unit cell angles	90.00, 113.52, 90.00

Refinement procedure

Resolution	34.372 - 1.882
R-factor	0.1479
Rwork	0.146
R-free	0.18650
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.837
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.372	1.970
High resolution limit [Å]	1.882	1.900
Number of reflections	97961	54651
<I/σ(I)>	14.3
Completeness [%]	99.4
Redundancy	5.8
CC(1/2)	0.839	0.839

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	1.6 M sodium citrate tri-basic dihydrate at pH 6.5