7XRF

Crystal structaure of DgpB/C complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-05-06
Detector	DECTRIS EIGER R 4M
Wavelength(s)	0.9875
Spacegroup name	P 21 21 21
Unit cell lengths	78.076, 159.704, 176.168
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.836 - 2.137
R-factor	0.2069
Rwork	0.206
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7exz
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.100
High resolution limit [Å]	2.100	2.100
Rmerge	0.200	0.860
Number of reflections	226255	689
<I/σ(I)>	6.3
Completeness [%]	99.9
Redundancy	14.5
CC(1/2)	0.860	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.2 M magnesium acetate and 10% PEG 8000