7XRA
Drimenyl diphosphate synthase D303A from Streptomyces showdoensis in complex with farnesyl diphosphate (FPP) and Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-01-15 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.97918 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 51.240, 113.620, 180.060 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.420 - 1.950 |
R-factor | 0.1794 |
Rwork | 0.177 |
R-free | 0.22210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7xqz |
Data reduction software | iMOSFLM |
Data scaling software | Aimless (0.7.7) |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 60.020 | 60.020 | 2.000 |
High resolution limit [Å] | 1.950 | 8.940 | 1.950 |
Rmerge | 0.111 | 0.034 | 0.558 |
Rmeas | 0.132 | 0.042 | 0.665 |
Rpim | 0.070 | 0.024 | 0.357 |
Total number of observations | 129439 | 1057 | 8898 |
Number of reflections | 38605 | 420 | 2675 |
<I/σ(I)> | 7.7 | 14.4 | 2.2 |
Completeness [%] | 99.5 | 93.4 | 99.9 |
Redundancy | 3.4 | 2.5 | 3.3 |
CC(1/2) | 0.993 | 0.998 | 0.762 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289.15 | HEPES, NaCl |