7XR7
Drimenyl diphosphate synthase D303E from Streptomyces showdoensis in complex with farnesyl diphosphate (FPP) and Mg2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-01-15 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.97918 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 51.640, 114.210, 180.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.430 - 1.630 |
R-factor | 0.1887 |
Rwork | 0.188 |
R-free | 0.21780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7xqz |
Data reduction software | iMOSFLM |
Data scaling software | Aimless (0.7.7) |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 57.100 | 57.100 | 1.660 |
High resolution limit [Å] | 1.630 | 8.930 | 1.630 |
Rmerge | 0.077 | 0.038 | 0.617 |
Rmeas | 0.091 | 0.052 | 0.725 |
Rpim | 0.049 | 0.036 | 0.375 |
Total number of observations | 218692 | 718 | 11379 |
Number of reflections | 66036 | 367 | 3279 |
<I/σ(I)> | 11.1 | 25.2 | 2.1 |
Completeness [%] | 98.8 | 81.2 | 99.8 |
Redundancy | 3.3 | 2 | 3.5 |
CC(1/2) | 0.993 | 0.941 | 0.745 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289.15 | HEPES, NaCl |