7XQC

Crystal structure of N-terminal domain of Rv2908c fused with Maltose Binding Protein (MBP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-05-17
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	68.860, 189.580, 72.130
Unit cell angles	90.00, 114.73, 90.00

Refinement procedure

Resolution	65.600 - 2.800
R-factor	0.1986
Rwork	0.196
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5iqz
RMSD bond length	0.007
RMSD bond angle	1.349
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	189.580	65.515	2.950
High resolution limit [Å]	2.800	8.850	2.800
Rmerge		0.034	0.548
Rmeas	0.260	0.047	0.739
Rpim	0.130	0.024	0.372
Total number of observations	162198	5264	23557
Number of reflections	41271	1346	6008
<I/σ(I)>	6	15.9	2.4
Completeness [%]	100.0	99.9	100
Redundancy	3.9	3.9	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	7.5	291.15	20 mM Na/K Phosphate, 20% (v/v) PEG 3350