7XQ5
Crystal structure of ScIno2p-ScIno4p bound promoter DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97847 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 97.893, 97.893, 89.780 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.950 - 2.250 |
R-factor | 0.2148 |
Rwork | 0.214 |
R-free | 0.23460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5gnj |
RMSD bond length | 0.008 |
RMSD bond angle | 1.045 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.950 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.073 | 1.592 |
Rmeas | 0.043 | 1.393 |
Rpim | 0.017 | 0.374 |
Number of reflections | 23995 | 2327 |
<I/σ(I)> | 30.3 | 2.4 |
Completeness [%] | 99.4 | 98.27 |
Redundancy | 19.4 | |
CC(1/2) | 1.000 | 0.769 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.01 M Cobalt (II) chloride hexahydrate, 0.1 M sodium acetate trihydrate, pH 5.0, 1.2 M 1, 6-hexandiol |