7XPT

Crystal structrue of MtdL:GDP:Mn soaked with GDP-Glc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	162.275, 98.825, 58.102
Unit cell angles	90.00, 96.77, 90.00

Refinement procedure

Resolution	57.700 - 2.000
R-factor	0.2174
Rwork	0.215
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xpr
Data reduction software	XDS
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.570	80.570	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.097	0.039	0.919
Rmeas	0.106	0.043	1.000
Rpim	0.041	0.017	0.388
Total number of observations	352445	4039	26413
Number of reflections	55455	708	4201
<I/σ(I)>	11.1	30.6	1.7
Completeness [%]	90.2	97.9	93.7
Redundancy	6.4	5.7	6.3
CC(1/2)	0.998	0.998	0.879

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		283	0.1 M tri-sodium citrate pH 5.6, 10% (w/v) PEG 4000, 10% (w/v) isopropanol