7XPN
Structure of the Spring Viraemia of Carp Virus Nucleoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 298.15 |
| Detector technology | CCD |
| Collection date | 2019-01-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 174.250, 298.700, 332.590 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.860 - 3.980 |
| R-factor | 0.2555 |
| Rwork | 0.255 |
| R-free | 0.28430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gic |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.260 | 4.122 |
| High resolution limit [Å] | 3.980 | 3.980 |
| Rmerge | 0.057 | 0.342 |
| Rmeas | 0.080 | 0.483 |
| Rpim | 0.057 | 0.342 |
| Number of reflections | 74437 | 7391 |
| <I/σ(I)> | 11.54 | 2.39 |
| Completeness [%] | 99.9 | 99.88 |
| Redundancy | 13.5 | 12.3 |
| CC(1/2) | 0.997 | 0.802 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIQUID DIFFUSION | 5.8 | 289.15 | 0.2 M Ammonium acetate, 0.1 M sodium citrate tribasic dihydrate pH 5.8, 21% v/v Polyethylene glycol 400 |
