7XPJ

crystal structure of rice ASI1 BAH domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 62 2 2
Unit cell lengths	107.327, 107.327, 73.362
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.983 - 2.301
R-factor	0.2231
Rwork	0.223
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3av4
RMSD bond length	0.002
RMSD bond angle	0.513
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.188	1.246
Number of reflections	11548	555
<I/σ(I)>	18.7	2.3
Completeness [%]	100.0	100
Redundancy	37.6	38.5
CC(1/2)	0.985	0.936

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	293	0.8 M ammonium sulfate and 0.1 M bicine, pH 9.0