7XPC
Complex structure of D-glycerate-3-kinase(GLYK) and AVRvnt1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 63 |
Unit cell lengths | 131.285, 131.285, 121.968 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 42.970 - 3.310 |
R-factor | 0.2956 |
Rwork | 0.293 |
R-free | 0.31720 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.970 | 42.970 | 3.500 |
High resolution limit [Å] | 3.310 | 9.710 | 3.310 |
Rmerge | 0.144 | 0.054 | 2.466 |
Rmeas | 0.152 | 0.057 | 2.595 |
Total number of observations | 363729 | ||
Number of reflections | 35167 | 1391 | 5663 |
<I/σ(I)> | 11.9 | 39.14 | 1.21 |
Completeness [%] | 99.9 | 98.7 | 99.7 |
Redundancy | 10.343 | 9.208 | 10.347 |
CC(1/2) | 0.998 | 0.998 | 0.576 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 277.15 | 10% w/v Polyethylene glycol 3,350, 0.1 M Tris pH 8.0, 100 mM Taurine and 10% w/v glycerol |