7XP0
Crystal structure of PmiR from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-10-18 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 74.352, 74.352, 188.238 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.380 - 2.610 |
R-factor | 0.2022 |
Rwork | 0.200 |
R-free | 0.25020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | model generated by Alphafold2 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 2.690 |
High resolution limit [Å] | 2.600 | 5.590 | 2.600 |
Rmerge | 0.115 | 0.102 | 0.434 |
Rmeas | 0.121 | 0.107 | 0.482 |
Rpim | 0.037 | 0.033 | 0.197 |
Total number of observations | 71992 | ||
Number of reflections | 8283 | 927 | 750 |
<I/σ(I)> | 15.1 | ||
Completeness [%] | 98.6 | 99.6 | 91.7 |
Redundancy | 8.7 | 10.7 | 4.6 |
CC(1/2) | 0.991 | 0.660 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | MES/Sodium hydroxide, Magnesium sulfate |