7XP0

Crystal structure of PmiR from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-10-18
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9793
Spacegroup name	I 41 2 2
Unit cell lengths	74.352, 74.352, 188.238
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.380 - 2.610
R-factor	0.2022
Rwork	0.200
R-free	0.25020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	model generated by Alphafold2
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.690
High resolution limit [Å]	2.600	5.590	2.600
Rmerge	0.115	0.102	0.434
Rmeas	0.121	0.107	0.482
Rpim	0.037	0.033	0.197
Total number of observations	71992
Number of reflections	8283	927	750
<I/σ(I)>	15.1
Completeness [%]	98.6	99.6	91.7
Redundancy	8.7	10.7	4.6
CC(1/2)		0.991	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	MES/Sodium hydroxide, Magnesium sulfate