7XOT
Crystal structure of Keap1_6k
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2022-03-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.771, 76.309, 48.248 |
| Unit cell angles | 90.00, 105.62, 90.00 |
Refinement procedure
| Resolution | 24.940 - 2.700 |
| R-factor | 0.1817 |
| Rwork | 0.176 |
| R-free | 0.23670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xmb |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.149 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (v1.17.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.119 | 0.521 |
| Number of reflections | 12109 | 1198 |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 98.6 | |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 4.0M Ammonium acetate, 0.1M Tris-HCl, pH 8.8. |
