7XND
Crystal structure of Phosphomevalonate kinase from Silkworm
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-10-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 54.070, 54.070, 123.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.480 - 1.600 |
| Rwork | 0.210 |
| R-free | 0.24680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ch4 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.645 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.480 | 1.640 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.066 | 1.236 |
| Rmeas | 0.068 | |
| Rpim | 0.320 | |
| Number of reflections | 24974 | 1817 |
| <I/σ(I)> | 27.1 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 23.2 | 15.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 289 | 1.8 M Ammonium citrate dibasic, 0.1 M Sodium acetate trihydrate pH 4.6 |
