7XMW

Crystal structure of anti-CRISPR protein AcrVIA2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2021-05-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97918
Spacegroup name	P 4 3 2
Unit cell lengths	123.377, 123.377, 123.377
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.130 - 2.590
R-factor	0.2543
Rwork	0.252
R-free	0.30670
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.077
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.130	2.683
High resolution limit [Å]	2.590	2.590
Number of reflections	9880	535
<I/σ(I)>	51.14
Completeness [%]	92.9
Redundancy	2
CC(1/2)	0.999	0.733

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIQUID DIFFUSION		291.15	TRIS hydrochloride, ammonium phosphate monobasic