7XMP
High resolution structure of lectin-like Ox-LDL receptor 1 in space group P 32 2 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 11.2C |
Synchrotron site | ELETTRA |
Beamline | 11.2C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-02-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 50.030, 50.030, 91.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.330 - 1.270 |
R-factor | 0.1598 |
Rwork | 0.158 |
R-free | 0.19720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ypu |
RMSD bond length | 0.015 |
RMSD bond angle | 1.674 |
Data reduction software | XDS (20210205) |
Data scaling software | Aimless |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 43.330 | 43.327 | 1.280 |
High resolution limit [Å] | 1.257 | 3.523 | 1.257 |
Rmerge | 0.098 | 0.071 | 1.641 |
Rmeas | 0.101 | 0.074 | 1.692 |
Rpim | 0.025 | 0.018 | 0.405 |
Total number of observations | 26662 | 30995 | |
Number of reflections | 36626 | 1832 | 1833 |
<I/σ(I)> | 15.4 | 32.2 | 1.8 |
Completeness [%] | 99.5 | 99.8 | 96.6 |
Redundancy | 16.7 | 14.6 | 16.9 |
CC(1/2) | 0.998 | 0.997 | 0.702 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 2M ammonium sulfate, 0.2 M sodium chloride, 0.1M sodium cacodylate |