7XLZ
Structure of siderophore-interacting protein from Vibrio anguillarum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-17 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97852 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.622, 58.466, 70.650 |
Unit cell angles | 90.00, 114.18, 90.00 |
Refinement procedure
Resolution | 58.951 - 1.190 |
R-factor | 0.139 |
Rwork | 0.138 |
R-free | 0.16150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gpj |
RMSD bond length | 0.008 |
RMSD bond angle | 1.032 |
Data reduction software | XDS (Jan 10, 2022, built on 20220220) |
Data scaling software | Aimless (version 0.5.29) |
Phasing software | PHASER (2.6.1) |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.951 | 1.194 |
High resolution limit [Å] | 1.190 | 1.190 |
Rmeas | 0.055 | 0.528 |
Rpim | 0.021 | 0.210 |
Number of reflections | 147256 | 1171 |
<I/σ(I)> | 20.2 | 3.3 |
Completeness [%] | 95.8 | 74.6 |
Redundancy | 6.7 | 6.1 |
CC(1/2) | 0.999 | 0.885 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | The crystal of VaSIP was grown in the drop containing 1.4 ul protein solution (60 mg/ml in 10 mM Hepes, 150 mM NaCl, pH 7.5, 1 mM DTT) and 1.4 ul reservoir solution (0.1 M KBr, 30% (W/V) mPEG 2000). |