7XL9

The structure of HucR with urate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-13
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.979
Spacegroup name	P 61 2 2
Unit cell lengths	44.730, 44.730, 283.280
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.980 - 2.580
R-factor	0.1901
Rwork	0.185
R-free	0.29459
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fbk
RMSD bond length	0.019
RMSD bond angle	2.125
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.000	2.650
High resolution limit [Å]	2.580	2.580
Rmeas	0.180
Number of reflections	5988	564
<I/σ(I)>	19.7
Completeness [%]	100.0
Redundancy	35.4
CC(1/2)	1.000	0.660

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	0.1 M Bis-Tris pH 7.0, 0.5 M MgCl2 and PEG 3350 20-30%