7XKC
Crystal structure of Daucus Carrot hypoglycemic peptide (DCHP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.850, 66.210, 121.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.780 - 2.560 |
R-factor | 0.2463 |
Rwork | 0.241 |
R-free | 0.28990 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3rdy |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.780 | 2.630 |
High resolution limit [Å] | 2.560 | 2.560 |
Rmerge | 0.200 | 0.605 |
Number of reflections | 14451 | 955 |
<I/σ(I)> | 14.8 | 2.4 |
Completeness [%] | 99.1 | 93.5 |
Redundancy | 9.5 | |
CC(1/2) | 0.975 | 0.597 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.2 M Sodium sulfate decahydrate, 20% (w/v) Polyethylene glycol (PEG) 3350 |