7XJV

Crystal Structure of Alpha-1,3-mannosyltransferase MNT2 from Saccharomyces cerevisiae, Mn/GDP-mannose form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	130.849, 131.189, 169.552
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.003 - 2.800
Rwork	0.213
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Predicted structure model by Alphafold
RMSD bond length	0.003
RMSD bond angle	1.204
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.970
High resolution limit [Å]	2.800	2.800
Number of reflections	139005	22509
<I/σ(I)>	7.7
Completeness [%]	99.7
Redundancy	6.9
CC(1/2)	0.988	0.947

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG 20000